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Quantification of 32P in bands after gel electrophoresis was performed using the flat-bed scintillation counter (Betaplate). The most convenient system involved placing fragments of dried gel between two glass fiber sheets, each previously sealed in a thin plastic bag with liquid scintillant. Good pulse-height spectra and counting efficiencies were obtained with low cross talk and background. The method has been used to quantify mRNA in RNA antisense-protection assays that were linear over a wide range (1-20000 cpm). Cross talk and background could be reduced further by an alternative technique utilizing plastic trays with shallow wells in which a solid scintillant had been melted. Fragments were immersed in the molten scintillant (90 degrees C), which was allowed to solidify, by cooling, before counting.

Type

Journal article

Journal

Anal Biochem

Publication Date

15/08/1991

Volume

197

Pages

121 - 124

Keywords

Animals, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Erythropoietin, Hypoxia, Kidney, Phosphorus Radioisotopes, RNA, Messenger, Rats, Scintillation Counting