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We have raised antisera and monoclonal antibodies (MAbs) to the C4 region of HIV-1 gp120, using an antigen chimaera of poliovirus as immunogen. These MAbs and sera, together with MAbs to the same region raised by other methods, fall into three groups defined by their abilities to bind to recombinant gp120 and/or the immunogenic peptide. In some cases, the amino acids recognized by the MAbs have been identified by pep-scan and by solution phase peptide inhibition of binding to recombinant gp120. Our results indicate that the amino acids WQEVGKAMYA are exposed on the surface of recombinant gp120. Antibodies to these amino acids on recombinant gp120 compete for soluble CD4 binding in vitro, but only weakly neutralize HIV.

Original publication

DOI

10.1089/aid.1992.8.451

Type

Journal article

Journal

AIDS Res Hum Retroviruses

Publication Date

04/1992

Volume

8

Pages

451 - 459

Keywords

Amino Acid Sequence, Antibodies, Monoclonal, Antigens, Viral, Base Sequence, Binding Sites, Binding, Competitive, CD4 Antigens, DNA, Viral, Epitopes, HIV Envelope Protein gp120, HIV-1, Molecular Sequence Data, Neutralization Tests, Poliovirus