Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.

Original publication

DOI

10.1073/pnas.0832180100

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

10/06/2003

Volume

100

Pages

7271 - 7276

Keywords

Antibodies, Monoclonal, Antigens, CD, Cell Line, Chimera, HIV, HeLa Cells, Hepacivirus, Hepatitis C Antibodies, Hepatocytes, Humans, Hydrogen-Ion Concentration, Membrane Proteins, Neutralization Tests, Receptors, Virus, Tetraspanin 28, Viral Envelope Proteins