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Short hairpin RNA (shRNA)-pooled screening is a valuable and cost-effective tool for assaying the contribution of individual genes to cell viability and proliferation on a genomic scale. Here we describe the key considerations for the design and execution of a pooled shRNA screen to identify determinants of radiosensitivity.

Original publication

DOI

10.1007/978-1-4939-6337-9_9

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2016

Volume

1470

Pages

103 - 119

Keywords

Genomic screening, Lentivirus, Pooled library, Radiosensitivity, shRNA, Anti-Bacterial Agents, Cell Count, Gene Library, Genomics, HEK293 Cells, Hexadimethrine Bromide, Humans, Polymerase Chain Reaction, RNA, Small Interfering, Radiation Tolerance