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5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA base modification in mammalian genomic DNA that is proposed to be a major epigenetic mark. We report here the syntheses of two new versions of phosphoramidites III and IV from 5-iodo-2'-deoxyuridine in 18% and 32% overall yields, respectively, with TBDMS as the 5-hydroxyl protecting group. Phosphoramidites III and IV allow efficient incorporation of 5-hmC into DNA and a "one-step" deprotection procedure to cleanly remove all the protecting groups. A "two-step" deprotection strategy is compatible with ultramild DNA synthesis, which enables the synthesis of 5hmC-containing DNA with additional modifications.

Original publication

DOI

10.1021/jo200566d

Type

Journal article

Journal

J Org Chem

Publication Date

20/05/2011

Volume

76

Pages

4182 - 4188

Keywords

5-Methylcytosine, Cytosine, DNA, Organophosphorus Compounds, Siloxanes, Styrenes