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Cell growth and proliferation are tightly linked to nutrient availability. The mechanistic target of rapamycin complex 1 (mTORC1) integrates the presence of growth factors, energy levels, glucose and amino acids to modulate metabolic status and cellular responses. mTORC1 is activated at the surface of lysosomes by the RAG GTPases and the Ragulator complex through a not fully understood mechanism monitoring amino acid availability in the lysosomal lumen and involving the vacuolar H(+)-ATPase. Here we describe the uncharacterized human member 9 of the solute carrier family 38 (SLC38A9) as a lysosomal membrane-resident protein competent in amino acid transport. Extensive functional proteomic analysis established SLC38A9 as an integral part of the Ragulator-RAG GTPases machinery. Gain of SLC38A9 function rendered cells resistant to amino acid withdrawal, whereas loss of SLC38A9 expression impaired amino-acid-induced mTORC1 activation. Thus SLC38A9 is a physical and functional component of the amino acid sensing machinery that controls the activation of mTOR.

Original publication

DOI

10.1038/nature14107

Type

Journal article

Journal

Nature

Publication Date

26/03/2015

Volume

519

Pages

477 - 481

Keywords

Amino Acid Transport Systems, Amino Acids, Animals, Cell Line, Humans, Lysosomes, Mechanistic Target of Rapamycin Complex 1, Mice, Monomeric GTP-Binding Proteins, Multiprotein Complexes, Nucleotides, TOR Serine-Threonine Kinases