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Methods to quantify cellular-level phenotypic differences between genetic groups are a key tool in genomics research. In disease processes such as cancer, phenotypic changes at the cellular level frequently manifest in the modification of cell population profiles. These changes are hard to detect due the ambiguity in identifying distinct cell phenotypes within a population. We present a methodology which enables the detection of such changes by generating a phenotypic signature of cell populations in a data-derived feature-space. Further, this signature is used to estimate a model for the redistribution of phenotypes that was induced by the genetic change. Results are presented on an experiment involving deletion of a tumor-suppressor gene dominant in breast cancer, where the methodology is used to detect changes in nuclear morphology between control and knockout groups.

Type

Journal article

Journal

Med Image Comput Comput Assist Interv

Publication Date

2011

Volume

14

Pages

343 - 351

Keywords

Algorithms, Animals, Breast Neoplasms, Cell Biology, Cell Nucleus, Cytological Techniques, Female, Fibroblasts, Humans, Image Processing, Computer-Assisted, Mice, Microscopy, Models, Theoretical, PTEN Phosphohydrolase, Phenotype