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We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.

Original publication

DOI

10.1128/JCM.01527-13

Type

Journal article

Journal

J Clin Microbiol

Publication Date

12/2013

Volume

51

Pages

4213 - 4216

Keywords

DNA, Protozoan, Genes, Protozoan, Genetic Markers, Molecular Sequence Data, Parasitology, Plasmodium ovale, Polymerase Chain Reaction, Sequence Analysis, DNA