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A monoclonal antibody, MHM.5, specific for HLA class I antigens, bound to lymphocytes of all donors tested and was thought to bind to a monomorphic determinant. When the antibody was used to precipitate 35S methionine labeled HLA class I molecules from lymphoid cells, which were then isoelectric focused, it was found that the HLA-A1,A2 and A3 antigens were not precipitated. Similarly, MHM.5, which is IgG1, failed to block complement mediated lysis by alloantisera specific for HLA-A1, 2 and 3, and most other HLA-A antigens. HLA-Aw24, A25, and A32, and all other HLA-B and C typing reactions tested were blocked. Thus the antibody binds to an epitope that is lacking on most A antigens, but present on Aw24, A25, A32 and all B and C locus antigens. Comparison of the published amino acid sequences of HLA-A2, A3, Aw24, A28, Cw3, B7, and B40 suggests some possible sites for this epitope.

Type

Journal article

Journal

Hum Immunol

Publication Date

05/1985

Volume

13

Pages

13 - 19

Keywords

Antibodies, Monoclonal, Antibody Specificity, Binding, Competitive, Epitopes, HLA Antigens, HLA-A Antigens, HLA-B Antigens, HLA-C Antigens, Humans, Isoelectric Focusing, Lymphocytes