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Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight.

Original publication

DOI

10.1002/eji.1830090307

Type

Journal article

Journal

Eur J Immunol

Publication Date

03/1979

Volume

9

Pages

205 - 210

Keywords

Animals, Antibodies, Antibody Specificity, Antigens, Binding Sites, Chemical Precipitation, Clone Cells, Electrophoresis, Polyacrylamide Gel, Female, Humans, Hybrid Cells, Hybridization, Genetic, Immunoenzyme Techniques, Mice, Mice, Inbred BALB C, Myeloma Proteins, T-Lymphocytes