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We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.

Original publication




Journal article


J Biomol NMR

Publication Date





133 - 137


Binding, Competitive, Humans, Ligands, Models, Molecular, Mutation, Nuclear Magnetic Resonance, Biomolecular, Protein Interaction Mapping, Reproducibility of Results, TCF Transcription Factors, Transcription Factor 7-Like 2 Protein, beta Catenin