Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.

Original publication

DOI

10.1007/s10858-007-9154-x

Type

Journal article

Journal

J Biomol NMR

Publication Date

06/2007

Volume

38

Pages

133 - 137

Keywords

Binding, Competitive, Humans, Ligands, Models, Molecular, Mutation, Nuclear Magnetic Resonance, Biomolecular, Protein Interaction Mapping, Reproducibility of Results, TCF Transcription Factors, Transcription Factor 7-Like 2 Protein, beta Catenin