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We have used Northern blot analysis to detect mRNA from class I HLA genes in the human choriocarcinoma cell line BeWo, which has been previously shown to express an atypical HLA class I molecule, in the absence of HLA A and B. Hybridization was seen with three class I DNA probes, the strongest being seen with the probe pC800, which corresponds to an 800-bp section of the Cw3 gene. We have made cDNA libraries from BeWo cells and screened for positive clones by using class I DNA probes. Of the clones isolated, we determined the complete sequence of one and partial sequence of five shorter clones. They all code for an identical C locus-related product, which does not correspond to published C locus sequences.

Type

Journal article

Journal

J Immunol

Publication Date

01/05/1989

Volume

142

Pages

3281 - 3285

Keywords

Amino Acid Sequence, Base Sequence, Blotting, Northern, Cell Line, Choriocarcinoma, Cloning, Molecular, DNA, DNA Probes, HLA, HLA-C Antigens, Humans, Molecular Sequence Data