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There are three prolyl hydroxylases (PHD1, 2 and 3) that regulate the hypoxia-inducible factors (HIFs), the master transcriptional regulators that respond to changes in intracellular O(2) tension. In high O(2) tension (normoxia) the PHDs hydroxylate two conserved proline residues on HIF-1α, which leads to binding of the von Hippel-Lindau (VHL) tumour suppressor, the recognition component of a ubiquitin-ligase complex, initiating HIF-1α ubiquitylation and degradation. However, it is not known whether PHDs and VHL act separately to exert their enzymatic activities on HIF-1α or as a multiprotein complex. Here we show that the tumour suppressor protein LIMD1 (LIM domain-containing protein) acts as a molecular scaffold, simultaneously binding the PHDs and VHL, thereby assembling a PHD-LIMD1-VHL protein complex and creating an enzymatic niche that enables efficient degradation of HIF-1α. Depletion of endogenous LIMD1 increases HIF-1α levels and transcriptional activity in both normoxia and hypoxia. Conversely, LIMD1 expression downregulates HIF-1 transcriptional activity in a manner depending on PHD and 26S proteasome activities. LIMD1 family member proteins Ajuba and WTIP also bind to VHL and PHDs 1 and 3, indicating that these LIM domain-containing proteins represent a previously unrecognized group of hypoxic regulators.

Original publication

DOI

10.1038/ncb2424

Type

Journal article

Journal

Nat Cell Biol

Publication Date

29/01/2012

Volume

14

Pages

201 - 208

Keywords

Cell Hypoxia, Cell Line, Tumor, HEK293 Cells, HeLa Cells, Humans, Hydroxylation, Hypoxia-Inducible Factor 1, alpha Subunit, Hypoxia-Inducible Factor-Proline Dioxygenases, Immunoblotting, Immunoprecipitation, Intracellular Signaling Peptides and Proteins, LIM Domain Proteins, Models, Biological, Polyubiquitin, Procollagen-Proline Dioxygenase, Proteasome Endopeptidase Complex, Protein Binding, RNA Interference, Transfection, Two-Hybrid System Techniques, Ubiquitination, Von Hippel-Lindau Tumor Suppressor Protein