Production and crystallization of MHC class I B allele single peptide complexes.
Reid SW., Smith KJ., Jakobsen BK., O'Callaghan CA., Reyburn H., Harlos K., Stuart DI., McMichael AJ., Bell JI., Jones EY.
Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P212121, with similar unit cell dimensions of approximately 52 A X 81 A X 112 A, contain one complex per asymmetric unit and diffract to approximately 2.0 A resolution.