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Deposits of Ig and complement are abundant in affected joints of patients with rheumatoid arthritis (RA) and in animal models of RA in which antibodies are demonstrably pathogenic. To identify molecular targets of the Igs deposited in arthritic joints, which may activate local inflammation, we used a combination of mass spectrometry (MS) and protein microarrays. Immune complexes were affinity-purified from surgically removed joint tissues of 26 RA and osteoarthritis (OA) patients. Proteins complexed with IgG were identified by proteomic analysis using tandem MS. A striking diversity of components of the extracellular matrix, and some intracellular components, copurified specifically with IgG from RA and OA tissues. A smaller set of autoantigens was observed only in RA eluates. In complementary experiments, IgG fractions purified from joint immune complexes were tested on protein microarrays against a range of candidate autoantigens. These Igs bound a diverse subset of proteins and peptides from synovium and cartilage, different from that bound by normal serum Ig. One type of intracellular protein detected specifically in RA joints (histones H2A/B) was validated by immunohistology and found to be deposited on the cartilage surface of RA but not OA joints. Thus, autoantibodies to many determinants (whether deposited as "neoantigens" or normal constituents of the extracellular matrix) have the potential to contribute to arthritic inflammation.

Original publication

DOI

10.1073/pnas.0908032106

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

15/09/2009

Volume

106

Pages

15867 - 15872

Keywords

Antibody Specificity, Antigen-Antibody Complex, Arthritis, Rheumatoid, Autoantibodies, Autoantigens, Case-Control Studies, Extracellular Matrix, Histones, Humans, Immunoglobulin G, Immunohistochemistry, Joints, Microscopy, Fluorescence, Osteoarthritis, Protein Array Analysis, Proteomics, Synovial Membrane, Tandem Mass Spectrometry