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Plasmodium falciparum-infected erythrocytes were metabolically labeled with tritiated glucosamine. Lipid extracts were analyzed by high-performance thin-layer chromatography to compare labeled molecules of eleven isolates from patients, six cytoadherent in vitro strains, and two knobbed and two knobless strains from Aotus monkeys. Up to nineteen labeled bands were identified. Glycolipid GL1, previously identified in Malayan Camp, was present in all isolates and strains. Other molecules, between CG and GM1 and between GM1 and GD1a, varied in mobility or presence. There was no apparent association between labeled molecules and the presence of knobs or the property of cytoadherence.

Type

Journal article

Journal

J Protozool

Publication Date

02/1988

Volume

35

Pages

169 - 172

Keywords

Animals, Aotus trivirgatus, Cell Adhesion, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Erythrocytes, Glycolipids, Humans, Phenotype, Plasmodium falciparum, Species Specificity