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The majority of HIV-1 infections worldwide occur in Africa, where subtype B viruses are rare and intersubtype recombinants are common. Pathogenesis and vaccine studies need to focus on viruses derived from African patients, and infectious HIV-1 molecular clones can be useful tools. To clone non-B subtypes and recombinant viruses from patients, we cultivated HIV-1 from the plasma of a Kenyan long-term survivor. Viral DNA was cloned into a plasmid, which was transfected into COS cells; progeny virus was propagated in PBMCs. Sequence analyses revealed that both the patient's plasma HIV-1 RNA and the cloned DNA genomes were recombinants between subtypes D and C; subtype C sequences comprised the nef and LTR regions. The cloned virus used the CCR5 coreceptor and did not form syncytia in vitro. This infectious HIV-1 subtype D/C recombinant molecular clone obtained from a Kenyan long-term survivor promises to be useful to study pathogenesis and vaccine design.

Original publication

DOI

10.1089/aid.2004.20.1015

Type

Journal article

Journal

AIDS Res Hum Retroviruses

Publication Date

09/2004

Volume

20

Pages

1015 - 1018

Keywords

Animals, COS Cells, Cercopithecus aethiops, Cloning, Molecular, DNA, Viral, Female, HIV Long-Term Survivors, HIV-1, Humans, Kenya, Molecular Sequence Data, Plasmids, Recombination, Genetic, Sequence Analysis, DNA, Transfection