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© 2018 Universiti Sains Malaysia. Using Saccharomyces cerevisiae lysate, two in-solution trypsin digestions (chloroform-methanol-water precipitation and RapiGest) were compared to the recently reported gel-aided sample preparation (GASP) workflow. Our proteomic results showed that GASP afforded the highest number of overall protein identifications and peptide spectrum matches without systematic bias towards peptide or protein size.

Original publication

DOI

10.21161/mjm.1461817

Type

Journal article

Journal

Malaysian Journal of Microbiology

Publication Date

01/01/2018

Volume

14

Pages

579 - 584