Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate
Yuan F., Bi Y., Siejka-Zielinska P., Zhou Y-L., Zhang X-X., Song C-X.
<p>WO-Seq: a bisulfite-free and base-resolution sequencing method based on peroxotungstate oxidation is presented for the identification of hm<sup>5</sup>C sites in the transcriptome. Combining the peroxotungstate oxidation with TET enzyme oxidation, m<sup>5</sup>C can also be detected in a procedure termed TET-Assisted WO-Seq (TAWO-Seq).</p>