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MotivationWith the continued improvement of requisite mass spectrometers and UHPLC systems, Hydrogen/Deuterium eXchange Mass Spectrometry (HDX-MS) workflows are rapidly evolving towards the investigation of more challenging biological systems, including large protein complexes and membrane proteins. The analysis of such extensive systems results in very large HDX-MS datasets for which specific analysis tools are required to speed up data validation and interpretation.ResultsWe introduce a web application and a new R-package named 'MEMHDX' to help users analyze, validate and visualize large HDX-MS datasets. MEMHDX is composed of two elements. A statistical tool aids in the validation of the results by applying a mixed-effects model for each peptide, in each experimental condition, and at each time point, taking into account the time dependency of the HDX reaction and number of independent replicates. Two adjusted P-values are generated per peptide, one for the 'Change in dynamics' and one for the 'Magnitude of ΔD', and are used to classify the data by means of a 'Logit' representation. A user-friendly interface developed with Shiny by RStudio facilitates the use of the package. This interactive tool allows the user to easily and rapidly validate, visualize and compare the relative deuterium incorporation on the amino acid sequence and 3D structure, providing both spatial and temporal information.Availability and implementationMEMHDX is freely available as a web tool at the project home page CONTACT: or sebastien.brier@pasteur.frSupplementary information: Supplementary data is available at Bioinformatics online.

Original publication




Journal article


Bioinformatics (Oxford, England)

Publication Date





3413 - 3419


Department of Chemistry and Structural Biology, Institut Pasteur, UMR CNRS, Paris 3528, France.


Hydrogen, Deuterium, Deuterium Exchange Measurement, Software, Mass Spectrometry, Datasets as Topic