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This chapter provides detailed methodology and materials required to profile deubiquitinases (DUBs) in a cellular matrix using specific activity-based probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying the potency and selectivity of DUB inhibitors are outlined here, including the standard ABPP, the deep DUBome ABPP, and the ABPP-HT (high-throughput compatible).

Original publication

DOI

10.1007/978-1-0716-2803-4_7

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2023

Volume

2591

Pages

101 - 122

Addresses

Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, UK.