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Mycoplasma Testing

There is a mycoplasma testing service offered by the NDM Research Building. Routine testing is scheduled for the first Thursday of each month (unless otherwise stated).

If you require the use of this service please fill in the request form below. An email confirming your request and the scheduled testing date will be sent on receipt of this form.

Requests must be received by Tuesday at 4pm to be tested the same week. Results are provided by the end of the day on a Friday.

Brief Outline

Testing is conducted using LONZA's MycoAlert Plus detection kit and BMG PHERASTAR FX multiplate reader. MycoAlert Plus is a selective biochemical luminescence assay that exploits the activity of certain mycoplasmal enzymes. The BMG luminescence protocol detects the increase (positive sample) or decrease (negative sample) of light produced during the breakdown of luciferin between the two readings. If you know your samples may contain luciferase or luciferin this test is not the correct way to test for mycoplasma.

You will need to provide 1mL of spun down supernatant (preferably in a 1.5ml tube) from >90% confluent cells. Please leave your samples in the cold room collection point on your floor. Supernatant needs to be fresh and ready to be tested on the morning of the scheduled test day.

Recharge cost

There is a set price for each sample and the positive and negative controls used during each test are divided equally between requestors that month. Charges are calculated and then recharged to your grant code at the end of the month. If you would like a break down of the cost please contact Chris Dodd who will outline how they are apportioned. If necessary, charges can be provided with your results.

 

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(Please include the name(s) of all sample(s) to be tested. Enter each name on a separate line)