Found 4248 matches for
Here, we present a protocol for lentiviral delivery of CRISPR-Cas9 to human induced pluripotent stem cell (iPSC)-derived macrophages using co-incubation with VPX virus-like particles (VPX-VLPs). We describe steps for producing polybrene and puromycin kill curves, VPX viral production, and VPX-VLP titration by western blotting. We then detail procedures for iPSC macrophage precursor lentiviral transduction and lentiviral CRISPR-Cas9-based knockout in iPSC-derived macrophages. This protocol uses efficient genome-editing techniques to explore macrophage involvement in immune response, chronic inflammation, neurodegenerative disease, and cancer progression. For complete details on the use and execution of this protocol, please refer to Navarro-Guerrero et al.1.
This book makes a forthright case for a shift in policy focus from 'community cohesion' to the broader notion of social cohesion, and is distinctive and innovative in its focus on evaluation. It constitutes an extremely valuable source both for practitioners involved in social cohesion interventions and for researchers and students studying theory-based evaluation and the policy areas highlighted (housing, intergenerational issues, the recession, education, communications, community development).
This paper explores the notion of 'ethnic group' focussing, in particular, on attempts to transform the concept into an empirical indicator in population censuses. The latter is seen to be riven with difficulties, not least the fact that such measures tend to be attempting to address two conflicting agendas - one requiring an ascriptive, the other a subjective, measure. Illustrating the core arguments with the decennial census in Britain, the paper explores the contested political terrain underpinning the introduction of such a question, and then demonstrates that the construction of an 'ethnic group' indicator takes the form of a complex dialectical process involving negotiation and re-negotiation on the part of a myriad of social actors and structural forces at macro-, meso- and micro-levels. Finally, it reflects on broader concerns arising from the reification of the measure, not least its material effects in the context of debates and policies on 'multiculturalism'. © 2013 Copyright Taylor and Francis Group, LLC.
The British government has instituted a wide range of educational reforms to tackle ethnic inequality. This article argues that over the past half century most of these have been driven by immediate political considerations and have failed to incorporate a broader, historical perspective. This has invariably led to short-term, and short cut, solutions to long-term, deeply entrenched problems that, in reality, transcend the world of education. The article evaluates all the major reform programs, ranging from assimilationism to multicultural education to anti-racist education. It concludes with discussion of the merits/demerits of faith schools and the use of school reorganization as a means of tackling ethnic segregation. © 2010 Elsevier Ltd. All rights reserved.
There is a wide swathe, and indeed long history, of UK literature featuring attempts to theorise differentials in housing position and shifting spatial settlement patterns in relation to ethnicity and 'race' (and also, more recently, faith group). Most of the earlier accounts were based on simplified versions of the structure-agency dualism or one or other variant of rational choice theory. Responding to criticisms that these relied too heavily on overly static notions of 'choice' and 'constraint', a few then turned to a form of theorisation that deployed a modified version of Giddens' structuration theory. This paper seeks to take the debate further by developing a model that retains much of the essence of structuration yet embodies a more dynamic and theoretically nuanced interpretation of both structure and agency. Structure, normally seen predominantly as a form of social regulation, will be seen as multi-layered and multi-dimensional and also, importantly, as subject to often unpredictable exogenous factors. The concept of social agency will also be subjected to a radical re-conceptualisation that reflects, amongst other things, recent shifts in social capital theory interpreted in the light of rapid demographic change (influenced by geo-political factors), ongoing social inequality, racism, and heightened inter- and intra-communal tensions in some areas. © 2009 Taylor & Francis.
This paper interrogates a concept at the core of a social policy agenda that has dominated thinking in the UK over the past decade. It argues that the notion of 'community cohesion' is based on a fundamentally flawed interpretation of the sources of tension and conflict in Britain's towns and cities. It overly ethnicizes societal divisions and essentializes ethnicity. Examining the development of government policy since 2001 the paper shows that the result has been a predominantly culturalist agenda that obscures key sources of division, most notably those related to social class and material inequality. It is argued that the hegemonic status of this policy stream has also undermined the equalities agenda. The paper concludes with a reflection on the implications of the emergence of a Conservative-led coalition government in May 2010. © The Author(s) 2012.
The concern of this article is with action at the local level to combat racial inequality in employment. It draws on the authors' evaluation of the 'West Midlands Common Standard', an innovative policy introduced by a consortium of West Midlands councils to ensure their contractors have, and implement, an equal opportunities in employment policy. The article assesses the impact of the initiative and its potential transferability. It is argued that the Common Standard provides a highly promising model for other local authorities to adopt.