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We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.

Original publication

DOI

10.1007/s10858-007-9154-x

Type

Journal article

Journal

J Biomol NMR

Publication Date

06/2007

Volume

38

Pages

133 - 137

Keywords

Binding, Competitive, Humans, Ligands, Models, Molecular, Mutation, Nuclear Magnetic Resonance, Biomolecular, Protein Interaction Mapping, Reproducibility of Results, TCF Transcription Factors, Transcription Factor 7-Like 2 Protein, beta Catenin